Cell Line Custom Development

EuroscreenFast offers you the possibility to benefit from our extensive cell line and assay development expertise to custom-engineer the tools expressing your target of choice, validated for functional or binding assay.

Our fully-integrated offer includes:

  • target sequence cloning in our high-copy bicistronic expression vector
  • transfection in the most appropriate host cell
  • selection of stable pool or clonal selection
  • characterization of the cell line using one of our available functional or binding readout
  • cell line can be transferred

 

Orthologue GPCR cell line development

Rhesus monkey CCR2 cell line development and characterization using an Aequorin (calcium) functional assay. Cell line was further validated in a binding assay.

Monkey CCR2 receptor agonist mode

Reference Compound Human CCR2 Monkey CCR2
MCP-1 EC50 (nM) 1.4 2.1
RS102895 IC50 (nM) 185 248

Non-GPCR cell line development

Transient Receptor Potential channel TRPA1 cell line was developed and characterized using an Aequorin (calcium) functional assay. Other non-GPCR targets include receptor tyrosine kinases (EGFR), ion channels (TRPV1, GRIN1), Sigma receptors (SigmaR1), enzymes (SRD5a)…

TRPA1 Aequorin agonist mode

We have successfully developed cell lines in these backgrounds:

  • CHO-K1
  • HEK293
  • 1321N1
  • U2OS
  • we’d love to try new ones….

If you can't find what you are looking for in our catalogue, let us know and we'll figure out how to develop it!